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首页> 外文OA文献 >Involvement of a Novel ABC Transporter and Monoalkyl Phthalate Ester Hydrolase in Phthalate Ester Catabolism by Rhodococcus jostii RHA1▿
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Involvement of a Novel ABC Transporter and Monoalkyl Phthalate Ester Hydrolase in Phthalate Ester Catabolism by Rhodococcus jostii RHA1▿

机译：新型ABC转运蛋白和邻苯二甲酸单烷基酯酯水解酶在邻苯二甲酸酯分解代谢中的作用Rhodococcus jostiiRHA1▿

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Phthalate esters (PEs) are important environmental pollutants. While the biodegradation of the parent compound, phthalate (PTH), is well characterized, the biodegradation of PEs is not well understood. In particular, prior to this study, genes involved in the uptake and hydrolysis of these compounds were not conclusively identified. We found that Rhodococcus jostii RHA1 could grow on a variety of monoalkyl PEs, including methyl, butyl, hexyl, and 2-ethylhexyl PTHs. Strain RHA1 could not grow on most dialkyl PEs, but suspensions of cells grown on PTH transformed dimethyl, diethyl, dipropyl, dibutyl, dihexyl and di-(2-ethylhexyl) PTHs. The major products of these dialkyl PEs were PTH and the corresponding monoalkyl PEs, and minor products resulted from the shortening of the alkyl side chains. RHA1 exhibited an inducible, ATP-dependent uptake system for PTH with a Km of 22 μM. The deletion and complementation of the patB gene demonstrated that the ATP-binding cassette (ABC) transporter encoded by patDABC is required for the uptake of PTH and monoalkyl PEs by RHA1. The hydrolase encoded by patE of RHA1 was expressed in Escherichia coli. PatE specifically hydrolyzed monoalkyl PEs to PTH but did not transform dialkyl PEs or other aromatic esters. This investigation of RHA1 elucidates key processes that are consistent with the environmental fate of PEs.
机译：邻苯二甲酸酯（PEs）是重要的环境污染物。尽管母体化合物邻苯二甲酸酯（PTH）的生物降解得到了很好的表征，但对PE的生物降解却知之甚少。特别是，在这项研究之前，尚未最终鉴定出涉及这些化合物摄取和水解的基因。我们发现红球菌RHA1可以在多种单烷基PE上生长，包括甲基，丁基，己基和2-乙基己基PTH。 RHA1菌株无法在大多数二烷基PE上生长，但是在PTH上生长的细胞悬浮液会转化为二甲基，二乙基，二丙基，二丁基，二己基和二-（2-乙基己基）PTH。这些二烷基PE的主要产物是PTH和相应的单烷基PE，而次要产物是由于烷基侧链的缩短。 RHA1表现出可诱导的ATP依赖型PTH摄取系统，Km为22μM。 patB基因的缺失和互补表明，patDABC编码的ATP结合盒（ABC）转运蛋白是RHA1摄取PTH和单烷基PE所必需的。由RHA1的patE编码的水解酶在大肠杆菌中表达。 PatE将单烷基PE特异性水解为PTH，但没有将二烷基PE或其他芳族酯转化。对RHA1的这项研究阐明了与PE的环境命运相一致的关键过程。

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